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KMID : 0363320140350030298
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Journal of Korean Oriental Internal Medicine
2014 Volume.35 No. 3 p.298 ~ p.308
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Protective Effects of Sosokmyoung-tang Against Parkinson's Model in Human Neuroblastoma SH-SY5Y Cells
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Woo Chan
You Ju-Yeon
Jang Chul-Yong
Kim Hyo-Rin
Shin Yong-Jeen
Moon A-Ji
Shin Sun-Ho
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Abstract
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Objectives: In this study we made an effort to investigate the protective effect of SSMT on the N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) -induced cytotoxicity of SH-SY5Y cells.
Methods: The cell viability was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MMT) assay. The fluorescence intensity was measured by using a dye and then with propidium iodide (PI) DNA flow cytometry analysis of the effects on the cell cycle of the SH-SY5Y cells and were used to measure the fluorescence of intracellular reactive oxygen species generation by MPTP.
Results: Pretreatment of SSMT significantly suppressed MPTP-induced cytotoxicity, which was revealed as apoptosis characterized by the reduction of cell viability, the increase of ROS production, and the loss of mitochondrial membrane potential in SH-SY5Y cells.
Conclusions: These findings suggest that SSMT exerts neuroprotective effects on human neuroblastoma SH-SY5Y cells by MPTP-induced dopaminergic neurodegeneration.
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KEYWORD
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SH-SY5Y cell, neuroprotective effect, Sosokmyoung-tang (SSMT)
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